Andreas Heuer
Research team manager
In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays
Author
Summary, in English
Direct electrochemical measurements of glutamate release in vivo were combined with optogenetics in order to examine light-induced control of glutamate neurotransmission in the rodent brain. Self-referenced recordings of glutamate using ceramic-based microelectrode arrays (MEAs) in hippocampus and frontal cortex demonstrated precise optical control of light-induced glutamate release through channelrhodopsin (ChR2) expression in both rat hippocampus and frontal cortex. Although the virus was only injected unilaterally, bilateral and rostro-caudal expression was observed in slice imaging, indicating diffusion and active transport of the viral particles. Methodology for the optogenetic control of glutamate signaling in the rat brain is thoroughly explained with special attention paid to MEA enzyme coating and cleaning for the benefit of other investigators. These data support that optogenetic control of glutamate signaling is robust with certain advantages as compared to other methods to modulate the in vivo control of glutamate signaling.
Department/s
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- Molecular Neurogenetics
- Developmental and Regenerative Neurobiology
- Department of Experimental Medical Science
Publishing year
2018
Language
English
Pages
327-351
Publication/Series
Neuromethods
Volume
130
Document type
Book chapter
Publisher
Humana Press
Topic
- Neurosciences
Keywords
- Amperometry
- Array
- Biosensor
- Electrochemistry
- Glutamate
- Glutamate oxidase
- Microelectrode
- Neurotransmitter
- Optogenetics
Status
Published
Research group
- Molecular Neurogenetics
- Developmental and Regenerative Neurobiology
ISBN/ISSN/Other
- ISSN: 19406045
- ISSN: 08932336