The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

MalinÅ

Malin Åkerblom

Project manager

MalinÅ

Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9.

Author

  • Malin Åkerblom
  • Rohit Sachdeva
  • Luis Quintino
  • Erika Elgstrand Wettergren
  • Katie Chapman
  • Giuseppe Manfre
  • Olle Lindvall
  • Cecilia Lundberg
  • Johan Jakobsson

Summary, in English

Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without affecting circulating and infiltrating monocytes or macrophages in an excitotoxic lesion model in rat striatum. The microRNA-9-regulated vectors described here are a straightforward and powerful tool that facilitates functional studies of resident microglia.

Department/s

  • Molecular Neurogenetics
  • CNS Gene Therapy
  • Neurology, Lund
  • StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
  • MultiPark: Multidisciplinary research focused on Parkinson´s disease

Publishing year

2013

Language

English

Publication/Series

Nature Communications

Volume

4

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Neurosciences

Status

Published

Research group

  • Molecular Neurogenetics
  • CNS Gene Therapy

ISBN/ISSN/Other

  • ISSN: 2041-1723